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Universal VMA in the sample or standard competes with a fixed amount of Universal VMA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VMA
Calculate the concentration of SAA2 in the sample by plotting standard curve
Substrate solution is then added and color develops in proportion to the amount of Human OCLN
Then Streptavidin-HRP is added and binds to the Biotinylated VWF antibody
Rabbit MYOG (Myogenin) ELISA Kit Packaging:48 wells Universal VMA in the sampleThe Rabbit MYOG (Myogenin) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rabbit MYOG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit MYOG and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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