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The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Mouse VEGF-A
Then Streptavidin-HRP is added and binds to the Biotinylated CFB antibody
The IL1F9 (Interleukin 1 Family
Rat CD200 (Ox-2 Membrane Glycoprotein) ELISA Kit Packaging:96 wells The concentration of Human ET-1The Rat (CD200) Ox 2 Membrane Glycoprotein ELISA Kit measures OX 2 membrane glycoprotein in Rat samples. The plate has been pre coated with Rat CD200 antibody. CD200 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CD200 Antibody is added and binds to CD200 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CD200 antibody. After incubation unbound Streptavidin HRP is washed
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