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Then a biotinylated detection antibody specific for Human CTLA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
When the sample containing HAI-1 is added to the plate
The concentration of SCF in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Mouse GAL9 in the samples is then determined by comparing the OD of the samples to the standard curve
tPA (Tissue Plasminogen Activator) ELISA Kit (Small Sample) Packaging:48 wells Then a biotinylated detection antibodyThe tPA (Tissue Plasminogen Activator) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human tPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human tPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
