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SDHA present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Human KLK10 Antibody is added and binds to KLK10 in the sample
it binds with HP bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated NDRG1 antibody
Human LIF (Leukemia inhibitory factor) ELISA Kit Packaging:96 wells SDHA present in the sampleThe Human LIF (Leukemia inhibitory factor) ELISA Kit measures LIF in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti LIF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
