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You can calculate the concentration of Rat FE in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-1Beta
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTGF
Rat LH (Luteinizing Hormone) ELISA Kit Packaging:96 wells You can calculate the concentrationThe Rat (LH) Luteinizing Hormone ELISA Kit measures Luteinizing Hormone in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added
