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SPA17 present in the sample is added and binds to antibodies coated on the wells
The concentration of Horse IFNg in the samples is then determined by comparing the OD of the samples to the standard curve
a capture antibody-TIMP-3-biotin-detection antibody complex was formed
a capture antibody-MAT2B-biotin-detection antibody complex was formed
Human AlphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit Packaging:48 wells SPA17 present in the sampleThe Human AlphaMSH (Alpha Melanocyte Stimulating Hormone) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human AlphaMSH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Human AlphaMSH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB
