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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 16 nm
Substrate solution is then added and color develops in proportion to the amount of Human AMTN
The concentration of Mouse GT in tested samples can be calculated by comparing the OD of the samples to the standard curve
And then biotinylated Human hnRNP A2B1 Antibody is added and binds to hnRNP A2B1 in the sample
Rat G6PD (Glucose-6-phosphate 1-dehydrogenase) ELISA Kit Packaging:96 wells The optical density (OD) isThe Rat (G6PD) Glucose 6 phosphate 1 dehydrogenase ELISA Kit measures Glucose 6 Phosphate 1 Dehydrogenase in Rat samples. The plate has been pre coated with Rat G6PDX antibody. G6PDX present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat G6PDX Antibody is added and binds to G6PDX in the sample. Then Streptavidin HRP is added and binds to the Biotinylated G6PDX antibody. After incubation unbound
