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The ACP3 (Acid Phosphatase 3
Substrate solution is then added and color develops in proportion to the amount of Human AQP2
a horseradish peroxidase conjugated mouse anti-Human IL-20 Antibody is added to the wells and binds to IL-20 in the sample
Then a biotinylated detection antibody specific for Rat GS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse F5 (Coagulation Factor 5) ELISA Kit Packaging:96 wells The ACP3 (Acid Phosphatase 3The Mouse (F5) Coagulation Factor 5 ELISA Kit measures coagulation factor 5 in Mouse samples. The plate has been pre coated with Mouse F5 antibody. F5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse F5 Antibody is added and binds to F5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated F5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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