Bovine CSF3 (Granulocyte Colony-stimulating Factor) ELISA Kit Packaging:96 wells Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCL2L11

Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Mouse β-EP in the sample or standard competes with a fixed amount of Mouse β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-EP

The OD value is proportional to the concentration of LIF

Bovine CSF3 (Granulocyte Colony-stimulating Factor) ELISA Kit Packaging:96 wells Standards or samples are addedThe Bovine (CSF3) Granulocyte Colony stimulating Factor ELISA Kit measures Granulocyte Colony Stimulating Factor in Bovine samples. The plate has been pre coated with Bovine CSF3 antibody. CSF3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine CSF3 Antibody is added and binds to CSF3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CSF3 antibody. After incubation unbound