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The absorbance is measured on a microplate reader at 450 nm
Then a biotinylated detection antibody specific for Mouse αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human LMF1 in the samples is then determined by comparing the OD of the samples to the standard curve
The assay plate has been pre-coated with mouse anti-Mouse Factor VII monoclonal antibody
MCPIP (Monocyte Chemoattractant Protein-induced Protein 1) ELISA Kit (Small Sample) Packaging:48 wells The absorbance is measured onThe MCPIP (Monocyte Chemoattractant Protein induced Protein 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human MCPIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human MCPIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
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