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Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTSZ
Then Streptavidin-HRP is added and binds to the Biotinylated ADAMTS19 antibody
You can calculate the concentration of Rat GAL1 in the samples by comparing the OD of the samples to the standard curve
Human FOLR1 (Folate Receptor Alpha) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (FOLR1) Folate Receptor Alpha ELISA Kit measures Folate Receptor Alpha in samples. The plate has been pre coated with Human FOLR1 antibody. FOLR1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FOLR1 Antibody is added and binds to FOLR1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FOLR1 antibody. After incubation unbound Streptavidin HRP is washed away