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Then a biotinylated detection antibody specific for Human sTfR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Egr2 present in the sample is added and binds to antibodies coated on the wells
Rat IL-1R2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat IL-1R2
The plate has been pre-coated with Human CYP11B1 antibody
Rat ANX-4 (Annexin 4) ELISA Kit PCOL-III Then a biotinylated detection antibodyThe Rat (ANX 4) Annexin 4 ELISA Kit measures annexin 4 in Rat samples. The plate has been pre coated with Rat ANX 4 antibody. ANX 4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ANX 4 Antibody is added and binds to ANX 4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ANX 4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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