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was isolated as a heterotetrameric fast axonal organelle transport motor consisting of 2 identical motor subunits (KHC) and 2 "light chains" (KLC) via microtubule affinity purification from neuronal cell extracts
You can calculate the concentration of Rat C3a in the samples by comparing the OD of the samples to the standard curve
coli expression system and the target gene encoding Ala20-Phe152 is expressed with a 6His tag at the N-terminus
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IL1a
Rat IL-23 ELISA Kit Packaging:48 wells was isolated as a heterotetramericThe Rat IL 23 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat IL23. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat IL23. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells
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