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TCF7 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Human HPRT1 antibody
The plate has been pre-coated with Human SEPHS2 antibody
Then a biotinylated detection antibody specific for Human FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human HHIP (Hedgehog Interacting Protein) ELISA Kit Packaging:96 wells TCF7 present in the sampleThe Human HHIP (Hedgehog Interacting Protein) ELISA Kit measures HHIP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HHIP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
