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The OD value is proportional to the concentration of Human TIMP-4
Then a biotinylated detection antibody specific for Human FGF18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
IL2RA present in the sample is added and binds to antibodies coated on the wells
The concentration of Human BMP3 in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse RSPO3 (R-Spondin-3) ELISA Kit Packaging:96 wells The OD value is proportionalThe Mouse (RSPO3) R Spondin 3 ELISA Kit measures R spondin 3 in Mouse samples. The plate has been pre coated with Mouse RSPO3 antibody. RSPO3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse RSPO3 Antibody is added and binds to RSPO3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RSPO3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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