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Then a biotinylated detection antibody specific for Human GCP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated DKKL1 antibody
The OD value is proportional to the concentration of Human IL-31
Then Streptavidin-HRP is added and binds to the Biotinylated CRHBP antibody
Human HTT (Huntingtin) ELISA Kit PSAP Then a biotinylated detection antibodyThe Human (HTT) Huntingtin ELISA Kit measures Huntingtin in samples. The plate has been pre coated with Human HTT antibody. HTT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HTT Antibody is added and binds to HTT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HTT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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