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After incubation, cells were pelleted (500 g, 5 min, 4 C) and the supernatant was filtered through a 5 m pore sterile filter (Sterile Millex Filter Unit, Millipore)
Raul is sadly missed
Conservatively, I would estimate the Curado DC 200 was achieving an extra 20 to 30 per cent in distance from the cheaper 150-sized reel being used in the back
She is survived by her granddaughter, Camryn Ruth Himel
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