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Mid-Season / Spawning Areas As the season progresses or you move to different venues, heavier gear is needed
With any kind of fishing, make sure your target species is able to ingest the hook and bait, says Gray
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At this stage, you can increase the drag from 2 to 4 pounds
For the control region, the protocol included an initial denaturation at 95 C for 5 min, followed by 38 cycles at 95 C for 20 s, 5055 C for 30 s, 72 C for 2 min and final extension at 72 C for 1 min
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