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When someone gets a spool all knotted up beyond saving, you replace it in two minutes and get back to fishing
I found that using a size 4 jawbone hook worked well with a Carolina rig for the fish I was after
The lines taper profile enhances delicate presentations and helps control the flys movement on the water
To asses cell viability, cells were labeled using the Fixable Viability Dye eFluor 780 (eBioscience/Thermo Fisher Scientific) as detailed in the manufacturers protocol, followed by flow cytometric (Cytoflex S, Beckmann Coulter) analysis of the samples
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